Target prediction and annotation of miRNA

•Collect the 3’UTR regions of all genes of Pengba.•Prepare the set of miRNA sequence list•Predict possible binding site by using tools like miRanda, RNA hybrid, Targetscan•For annotation, take the target gene list and perform BLASTx against Uniprot/NCBI NR to identify gene name.•For mapping the genes use GO to understand functions of the genes regulated by miRNA.•To know the biological pathway KEGG analysis will be used.